Inter-rater reliability of phenotypes and exploratory genotype-phenotype analysis in inherited hidradenitis suppurativa.

Genotype-phenotype correlation measures the correlation between the presence of a bodily trait with a bunch of comparable mutations however depends on dependable phenotyping.

It can present info on illness pathogenesis, future illness development, severity or exercise. Such indicators could be invaluable in hidradenitis suppurativa (HS).To assess inter-rater reliability (IRR) of HS scientific phenotypes and carry out exploratory genotype-phenotype correlation in circumstances of HS with recognized sequence variants.

METHODSLinkage disequilibrium between variants was assessed. Genotype-phenotype correlations have been explored utilizing Spearman correlation coefficients. IRR was calculated utilizing Cohen’s κ. Correlation between phenotype classifications was assessed utilizing the χ² statistic.

RESULTSForty-three sequence variants with scientific info have been recognized. Clinical phenotypes have been labeled as LC2 (n = 29; 67%), scarring folliculitis (n = 18; 42%), atypical (n = 38; 88%) and nodular (n = 26; 60%). LC1 phenotype was related to common (χ² = 41·289, P < 0·001) and typical (χ² = 29·013, P < 0·001) phenotypes. Cohen’s κ was highest for van der Zee and Jemec (0·815), adopted by Martorell-Calatayud et al. (0·813), Naasan and Affleck (0·774) and Canoui-Poitrine et al. (0·435) classifications.

NFRKB
MBS8563149-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

NFRKB
MBS8563149-02mL	MyBiosource	0.2mL	EUR 345

NFRKB Antibody
8C17081	AAT Bioquest	50ug	EUR 368
Description: NFRKB Antibody

NFRKB antibody
70R-50128	Fitzgerald	100 ul	EUR 242
Description: Purified Polyclonal NFRKB antibody

NFRKB antibody
10R-1483	Fitzgerald	50 ug	EUR 290.4
Description: Mouse monoclonal NFRKB antibody

NFRKB Antibody
1-CSB-PA003423	Cusabio	Ask for price Ask for price	50ug 100ug
Description: A polyclonal antibody against NFRKB. Recognizes NFRKB from Human, Mouse, Monkey. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000

NFRKB Antibody
AF9129	Affbiotech	200ul	EUR 420

NFRKB Antibody
AF9129-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IF/ICC,ELISA(peptide)

NFRKB Antibody
AF9129-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IF/ICC,ELISA(peptide)

NFRKB Antibody
GWB-ML805D	GenWay Biotech	50ug	Ask for price

NFRKB Antibody
E11-17081C	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

NFRKB Antibody
E311019	EnoGene	200ul	EUR 275
Description: Available in various conjugation types.

NFRKB Antibody
E38PA1809	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

NFRKB Antibody
MBS5314748-01mL	MyBiosource	0.1mL	EUR 470

NFRKB Antibody
MBS5314748-5x01mL	MyBiosource	5x0.1mL	EUR 1955

NFRKB Antibody
MBS7122208-005mg	MyBiosource	0.05mg	EUR 150

NFRKB Antibody
MBS7122208-01mg	MyBiosource	0.1mg	EUR 190

NFRKB Antibody
MBS7122208-5x01mg	MyBiosource	5x0.1mg	EUR 845

NFRKB Antibody
MBS8580863-01mL	MyBiosource	0.1mL	EUR 305

NFRKB Antibody
MBS8580863-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

NFRKB Antibody
MBS8580863-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

NFRKB Antibody
MBS8580863-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

NFRKB Antibody
MBS8580863-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

NFRKB Antibody
MBS8509893-01mg	MyBiosource	0.1mg	EUR 305

NFRKB Antibody
MBS8509893-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

NFRKB Antibody
MBS8509893-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

NFRKB Antibody
MBS8509893-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

NFRKB Antibody
MBS8509893-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

NFRKB Antibody
MBS9506620-005mL	MyBiosource	0.05mL	EUR 285

NFRKB Antibody
MBS9506620-01mL	MyBiosource	0.1mL	EUR 385

NFRKB Antibody
MBS9506620-5x01mL	MyBiosource	5x0.1mL	EUR 1590

NFRKB Rabbit mAb
A23915	Abclonal	100μL	EUR 138.16
Description: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2ERK1/2 (P27361/P28482).

NFRKB Rabbit pAb
A10124	Abclonal	100μL	EUR 70.85

NFRKB Rabbit pAb
A10124-100ul	Abclonal	100 ul	EUR 369.6

NFRKB Rabbit pAb
A10124-200ul	Abclonal	200 ul	EUR 550.8

NFRKB Rabbit pAb
A10124-20ul	Abclonal	20 ul	EUR 219.6

NFRKB Rabbit pAb
A10124-50ul	Abclonal	50 ul	EUR 267.6

NFRKB cDNA Clone
MBS1277284-001mgPlasmid02mLGlycerolStock	MyBiosource	0.01mgPlasmid+0.2mLGlycerol-Stock	EUR 1355

NFRKB cDNA Clone
MBS1277284-5x001mgPlasmid5x02mLGlycerolStock	MyBiosource	5x0.01mgPlasmid+5x0.2mLGlycerol-Stock	EUR 6050

NFRKB Rabbit pAb
E45R17753N	EnoGene	50 ul	EUR 297.75

Mouse NFRKB siRNA
20-abx925841	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

Human NFRKB siRNA
20-abx925842	Abbexa	Ask for price Ask for price	15 nmol 30 nmol

NFRKB siRNA (Mouse)
MBS829470-15nmol	MyBiosource	15nmol	EUR 405

NFRKB siRNA (Mouse)
MBS829470-30nmol	MyBiosource	30nmol	EUR 565

NFRKB siRNA (Mouse)
MBS829470-5x30nmol	MyBiosource	5x30nmol	EUR 2450

NFRKB siRNA (Human)
MBS8211045-15nmol	MyBiosource	15nmol	EUR 405

NFRKB siRNA (Human)
MBS8211045-30nmol	MyBiosource	30nmol	EUR 565

NFRKB siRNA (Human)
MBS8211045-5x30nmol	MyBiosource	5x30nmol	EUR 2450

NFRKB cloning plasmid
CSB-CL757573HU-10ug	Cusabio	10ug	EUR 1644
Description: A cloning plasmid for the NFRKB gene.

NFRKB Blocking Peptide
20-abx063003	Abbexa	Ask for price Ask for price	1 mg 5 mg

NFRKB Blocking Peptide
AF9129-BP	Affbiotech	1mg	EUR 234

NFRKB Blocking Peptide
MBS823650-1mg	MyBiosource	1mg	EUR 190

NFRKB Blocking Peptide
MBS823650-5mg	MyBiosource	5mg	EUR 345

NFRKB Blocking Peptide
MBS823650-5x5mg	MyBiosource	5x5mg	EUR 1465

NFRKB Blocking Peptide
MBS9619626-1mg	MyBiosource	1mg	EUR 380

NFRKB Blocking Peptide
MBS9619626-5x1mg	MyBiosource	5x1mg	EUR 1650

NFRKB Polyclonal Antibody
27297-100ul	SAB	100ul	EUR 302.4

NFRKB Polyclonal Antibody
27297-50ul	SAB	50ul	EUR 224.4

NFRKB Polyclonal Antibody
E-AB-90081-120uL	Elabscience Biotech	120uL	EUR 320
Description: Unconjugated

NFRKB Polyclonal Antibody
E-AB-90081-200uL	Elabscience Biotech	200uL	EUR 530
Description: Unconjugated

NFRKB Polyclonal Antibody
E-AB-90081-60uL	Elabscience Biotech	60uL	EUR 200
Description: Unconjugated

NFRKB Polyclonal Antibody
E-AB-90081-each	Elabscience Biotech	each	Ask for price
Description: Unconjugated

NFRKB polyclonal antibody
BS74375	Bioworld Biotech	50ul	EUR 358
Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

NFRKB Polyclonal Antibody
E910124	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

NFRKB Polyclonal Antibody
MBS9129762-002mL	MyBiosource	0.02mL	EUR 200

NFRKB Polyclonal Antibody
MBS9129762-005mL	MyBiosource	0.05mL	EUR 255

NFRKB Polyclonal Antibody
MBS9129762-01mL	MyBiosource	0.1mL	EUR 345

NFRKB Polyclonal Antibody
MBS9129762-02mL	MyBiosource	0.2mL	EUR 545

NFRKB Polyclonal Antibody
MBS9129762-5x02mL	MyBiosource	5x0.2mL	EUR 2265

NFRKB Polyclonal Antibody
MBS8566289-01mL	MyBiosource	0.1mL	EUR 305

NFRKB Polyclonal Antibody
MBS8566289-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

NFRKB Polyclonal Antibody
MBS8566289-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

NFRKB Polyclonal Antibody
MBS8566289-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

NFRKB Polyclonal Antibody
MBS8566289-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

NFRKB Polyclonal Antibody
MBS9431173-005mL	MyBiosource	0.05mL	EUR 300

NFRKB Polyclonal Antibody
MBS9431173-01mL	MyBiosource	0.1mL	EUR 390

NFRKB Polyclonal Antibody
MBS9431173-5x01mL	MyBiosource	5x0.1mL	EUR 1610

Mouse Nfrkb ELISA KIT
ELI-39529m	Lifescience Market	96 Tests	EUR 1038

Human NFRKB ELISA KIT
ELI-44624h	Lifescience Market	96 Tests	EUR 988.8

Mouse NFRKB shRNA Plasmid
20-abx982184	Abbexa	Ask for price Ask for price	150 µg 300 µg

Human NFRKB shRNA Plasmid
20-abx953184	Abbexa	Ask for price Ask for price	150 µg 300 µg

Human NFRKB Protein Lysate
MBS8415999-002mg	MyBiosource	0.02mg	EUR 545

Human NFRKB Protein Lysate
MBS8415999-5x002mg	MyBiosource	5x0.02mg	EUR 2225

NFRKB (NM_006165) Human Over-expression Lysate
E45H08062-2	EnoGene	each	EUR 395

NFRKB (NM_006165) Human Over-expression Lysate
LS004798	BosterBio	100ug	EUR 960
Description: Transient overexpression lysate of nuclear factor related to kappaB binding protein (NFRKB), transcript variant 2

Nfrkb (GFP-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb)
MG211898	Origene Technologies GmbH	10 µg	Ask for price

Nfrkb (untagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb), (10ug)
MC205447	Origene Technologies GmbH	10 µg	Ask for price

Recombinant Human NFRKB Protein
E30P69630A	EnoGene	50 ug	EUR 295

Nfrkb ORF Vector (Rat) (pORF)
ORF071281	ABM	1.0 ug DNA	EUR 607.2

NFRKB ORF Vector (Human) (pORF)
ORF007055	ABM	1.0 ug DNA	EUR 114

Nfrkb ORF Vector (Mouse) (pORF)
ORF051325	ABM	1.0 ug DNA	EUR 607.2

NFRKB Polyclonal Conjugated Antibody
C27297	SAB	100ul	EUR 476.4

NFRKB Polyclonal Conjugated Antibody
MBS9439188-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

NFRKB Polyclonal Conjugated Antibody
MBS9439188-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

NFRKB Polyclonal Conjugated Antibody
MBS9439188-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

NFRKB Polyclonal Conjugated Antibody
MBS9439188-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

NFRKB Polyclonal Conjugated Antibody
MBS9439188-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

Nfrkb (Myc-DDK-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb)
MR211898	Origene Technologies GmbH	10 µg	Ask for price

Human NFRKB knockout cell line
ABC-KH10074	AcceGen	1 vial	Ask for price
Description: Human NFRKB knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Nfrkb (untagged ORF) - Rat nuclear factor related to kappa B binding protein (Nfrkb), (10 ug)
RN213386	Origene Technologies GmbH	10 µg	Ask for price

Human NFRKB knockdown cell line
ABC-KD10074	AcceGen	1 vial	Ask for price
Description: Human NFRKB knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human NFRKB Protein Lysate 20ug
IHUNFRKBPLLY20UG	Innovative research	each	EUR 361
Description: Human NFRKB Protein Lysate 20ug

NFRKB ELISA Kit (Human) (OKCD01959)
OKCD01959	Aviva Systems Biology	96 Wells	EUR 997.2
Description: Description of target: Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.127 ng/mL

Anti-NFR kappaB NFRKB Antibody
A06867	BosterBio	100ul	EUR 399
Description: Boster Bio Anti-NFR kappaB NFRKB Antibody catalog # A06867. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.

NFRKB Peptide - N-terminal region
MBS3226949-01mg	MyBiosource	0.1mg	EUR 180

NFRKB Peptide - N-terminal region
MBS3226949-5x01mg	MyBiosource	5x0.1mg	EUR 730

NFRKB antibody - N-terminal region
MBS3201953-01mL	MyBiosource	0.1mL	EUR 455

NFRKB antibody - N-terminal region
MBS3201953-5x01mL	MyBiosource	5x0.1mL	EUR 1995

NFRKB (untagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 2
SC309921	Origene Technologies GmbH	10 µg	Ask for price

NFRKB (untagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 1
SC325410	Origene Technologies GmbH	10 µg	Ask for price

OCOA12249-20UG - NFRKB Protein Lysate
OCOA12249-20UG	Aviva Systems Biology	20ug	EUR 269

NFRKB (GFP-tagged) - Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 2
RG215038	Origene Technologies GmbH	10 µg	Ask for price

NFRKB (GFP-tagged) - Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 1
RG226572	Origene Technologies GmbH	10 µg	Ask for price

Nfrkb (Myc-DDK-tagged ORF) - Rat nuclear factor related to kappa B binding protein (Nfrkb), (10 ug)
RR213386	Origene Technologies GmbH	10 µg	Ask for price

OKCD01959-96W - NFRKB ELISA Kit (Human)
OKCD01959-96W	Aviva Systems Biology	96Wells	EUR 675

Lenti ORF clone of Nfrkb (mGFP-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb)
MR211898L4	Origene Technologies GmbH	10 µg	Ask for price

Nfrkb 3'UTR GFP Stable Cell Line
TU263943	ABM	1.0 ml	Ask for price

Nfrkb 3'UTR GFP Stable Cell Line
TU164049	ABM	1.0 ml	Ask for price

NFRKB 3'UTR GFP Stable Cell Line
TU065643	ABM	1.0 ml	EUR 1825.2

Nfrkb sgRNA CRISPR Lentivector set (Rat)
K6156601	ABM	3 x 1.0 ug	EUR 406.8

NFRKB (Myc-DDK-tagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 2
RC215038	Origene Technologies GmbH	10 µg	Ask for price

NFRKB (Myc-DDK-tagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 1
RC226572	Origene Technologies GmbH	10 µg	Ask for price

NFRKB sgRNA CRISPR Lentivector set (Human)
K1423401	ABM	3 x 1.0 ug	EUR 406.8

Nfrkb sgRNA CRISPR Lentivector set (Mouse)
K3738301	ABM	3 x 1.0 ug	EUR 406.8

NFRKB Protein Vector (Rat) (pPM-C-HA)
PV285124	ABM	500 ng	EUR 1429.2

Lenti ORF clone of Nfrkb (Myc-DDK-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb)
MR211898L3	Origene Technologies GmbH	10 µg	Ask for price

NFRKB Protein Vector (Rat) (pPB-C-His)
PV285122	ABM	500 ng	EUR 1429.2

NFRKB Protein Vector (Rat) (pPB-N-His)
PV285123	ABM	500 ng	EUR 1429.2

NFRKB Protein Vector (Rat) (pPM-C-His)
PV285125	ABM	500 ng	EUR 1429.2

Lenti ORF particles, Nfrkb (GFP-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb), 200ul, >10^7 TU/mL
MR211898L4V	Origene Technologies GmbH	200 µl	Ask for price

NFRKB Protein Vector (Human) (pPM-C-HA)
PV028219	ABM	500 ng	EUR 394.8

NFRKB Protein Vector (Mouse) (pPM-C-HA)
PV205300	ABM	500 ng	EUR 1278

NFRKB Protein Vector (Human) (pPB-C-His)
PV028217	ABM	500 ng	EUR 394.8

NFRKB Protein Vector (Human) (pPB-N-His)
PV028218	ABM	500 ng	EUR 394.8

NFRKB Protein Vector (Human) (pPM-C-His)
PV028220	ABM	500 ng	EUR 394.8

NFRKB Protein Vector (Mouse) (pPB-C-His)
PV205298	ABM	500 ng	EUR 1278

NFRKB Protein Vector (Mouse) (pPB-N-His)
PV205299	ABM	500 ng	EUR 1278

NFRKB Protein Vector (Mouse) (pPM-C-His)
PV205301	ABM	500 ng	EUR 1278

Nfrkb 3'UTR Luciferase Stable Cell Line
TU114049	ABM	1.0 ml	Ask for price

Nfrkb 3'UTR Luciferase Stable Cell Line
TU213943	ABM	1.0 ml	Ask for price

NFRKB 3'UTR Luciferase Stable Cell Line
TU015643	ABM	1.0 ml	EUR 1825.2

Lenti ORF particles, Nfrkb (Myc-DDK-tagged) - Mouse nuclear factor related to kappa B binding protein (Nfrkb), 200ul, >10^7 TU/mL
MR211898L3V	Origene Technologies GmbH	200 µl	Ask for price

Nfrkb sgRNA CRISPR Lentivector (Rat) (Target 1)
K6156602	ABM	1.0 ug DNA	EUR 184.8

Nfrkb sgRNA CRISPR Lentivector (Rat) (Target 2)
K6156603	ABM	1.0 ug DNA	EUR 184.8

Nfrkb sgRNA CRISPR Lentivector (Rat) (Target 3)
K6156604	ABM	1.0 ug DNA	EUR 184.8

NFRKB sgRNA CRISPR Lentivector (Human) (Target 1)
K1423402	ABM	1.0 ug DNA	EUR 184.8

NFRKB sgRNA CRISPR Lentivector (Human) (Target 2)
K1423403	ABM	1.0 ug DNA	EUR 184.8

NFRKB sgRNA CRISPR Lentivector (Human) (Target 3)
K1423404	ABM	1.0 ug DNA	EUR 184.8

Nfrkb sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3738302	ABM	1.0 ug DNA	EUR 184.8

Nfrkb sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3738303	ABM	1.0 ug DNA	EUR 184.8

Nfrkb sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3738304	ABM	1.0 ug DNA	EUR 184.8

Lenti-ORF clone of NFRKB (mGFP-tagged)-Human nuclear factor related to kappaB binding protein (NFRKB), transcript variant 1
RC226572L4	Origene Technologies GmbH	10 µg	Ask for price

AAP34265-100UG - NFRKB Peptide - N-terminal region
AAP34265-100UG	Aviva Systems Biology	100ug	EUR 99

ARP34265_P050-25UL - NFRKB Antibody - N-terminal region
ARP34265_P050-25UL	Aviva Systems Biology	25ul	EUR 99

High linkage disequilibrium was seen between variants of Han Chinese pedigrees. No important genotype-phenotype correlations have been recognized.These findings could also be influenced by choice, publication bias and the belief that HS is a monogenic dysfunction.

The poor IRR of present phenotype measures suggests restricted utility of present measures. Further investigations into the correlation of scientific phenotypes with inflammatory biomarkers could help in prognostic efforts for this illness.

What’s already identified about this subject? Genotype-phenotype correlation can present info concerning illness pathogenesis and predictions for future illness development, severity or exercise. The identification of such indicators in hidradenitis suppurativa (HS) could be invaluable for sufferers and clinicians alike, given the shortage of biomarkers or scientific predictors of illness.

What does this research add? Sixty-five sequence variants throughout 20 separate genes have been recognized. There was no important correlation between phenotype classification in 4 separate classification schema and gene, mutation kind or impression on Notch signalling.

Utility of present phenotype measurements are restricted. The lack of genotype-phenotype correlation in HS is suggestive that the underlying assumption of inherited HS as a monogenic dysfunction may have revision.