Improved transport system for Neisseria gonorrhoeae in clinical specimens.

Improved transport system for Neisseria gonorrhoeae in clinical specimens.

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Protective transport media have for use to protect Neisseria gonorrhoeae in clinical specimens throughout their transit to the laboratory. In this examine, a CO2- setting chamber, the Jembec chamber, was used for transport of clinical speciments requiring examination for gonococci.

The survival of N. gonorrhoeae current in clinical speciments when positioned in Amies charcoal transport medium was in comparison with their survival when inoculated into Jembec chambers containing both modified Thayer-Martin medium (MTM) or modified New York City transport medium (MNYC).

For a interval of as much as 2 days in transit, the three methods weren’t considerably totally different. However, after three days in transit, MNYC/Jembec chambers preserved considerably extra gonococci than Amies charcoal transport meduim (P lower than 0.0001) or MTM/Jembec chambers (P=0.006). MNYC/Jembec chambers withstood 241 miles (386 km) of postal transit throughout winter months; 80% of the gonococci current in clinical specimens remained viable from 2 to five days below these situations.

Improved transport system for Neisseria gonorrhoeae in clinical specimens.
Improved transport system for Neisseria gonorrhoeae in clinical specimens.

The CO2 generated by the pill in the Jembec chamber was suggicient to help the expansion of N. gonorrhoeae if the chambers had been incubated at 36 C instantly after inoculation.

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However, if delayed in transit, the chambers needed to be incubated in 5 to 10% CO2 to advertise the expansion of N. gonorrhoeae. MNYC/Jembec chambers present a selective setting that can defend and keep the viability of N. gonorrhoeae for prolonged durations, permitting an affordable time for postal transit of clinical specimens to the laboratory.

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