Protective transport media have for use to protect Neisseria gonorrhoeae in clinical specimens throughout their transit to the laboratory. In this examine, a CO2- setting chamber, the Jembecchamber, was used for transport of clinical speciments requiring examination for gonococci.
The survival of N. gonorrhoeae current in clinical speciments when positioned in Amies charcoal transport medium was in comparison with their survival when inoculated into Jembec chambers containing both modified Thayer-Martin medium (MTM) or modified New York City transport medium (MNYC).
For a interval of as much as 2 days in transit, the three methods weren’t considerably totally different. However, after three days in transit, MNYC/Jembec chambers preserved considerably extra gonococci than Amies charcoal transport meduim (P lower than 0.0001) or MTM/Jembec chambers (P=0.006). MNYC/Jembec chambers withstood 241 miles (386 km) of postal transit throughout winter months; 80% of the gonococci current in clinical specimens remained viable from 2 to five days below these situations.
Improved transport system for Neisseria gonorrhoeae in clinical specimens.
The CO2 generated by the pill in the Jembec chamber was suggicient to help the expansion of N. gonorrhoeae if the chambers had been incubated at 36 C instantly after inoculation.
Description: A polyclonal antibody against NFYB. Recognizes NFYB from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NFYB . This antibody is tested and proven to work in the following applications:
Description: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
Description: Description of target: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Qualitative DNA Binding ELISA ;Sensitivity:
Description: A Monoclonal antibody against Human NFYB (monoclonal) (M01). The antibodies are raised in mouse and are from clone 6H6. This antibody is applicable in WB, E
Description: A Monoclonal antibody against Human NFYB (monoclonal) (M03). The antibodies are raised in mouse and are from clone 5D1. This antibody is applicable in WB and IF, E
However, if delayed in transit, the chambers needed to be incubated in 5 to 10% CO2 to advertise the expansion of N. gonorrhoeae. MNYC/Jembec chambers present a selective setting that can defend and keep the viability of N. gonorrhoeae for prolonged durations, permitting an affordable time for postal transit of clinical specimens to the laboratory.
