Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

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A prototype, nonisotopic, chemiluminescent DNA probe test referred to as the Gen-Probe PACE (Probe Assay-Chemiluminescence Enhanced) system for Neisseria gonorrhoeae (Gen-Probe, San Diego, Calif.) was in contrast with standard Martin-Lewis tradition medium in JEMBEC plates for the laboratory diagnosis of gonorrhea.

This 2-h noncultural assay is predicated upon the use of an acridinium ester-labeled DNA probe. The rRNA-directed DNA probe hybridizes with the goal rRNA, and the hybridized probe is separated from the unhybridized probe by means of the use of magnetic microparticles.

The esterified acridinium is hydrolyzed from the hybridized probe by the addition of an alkaline hydrogen peroxide resolution, leading to the manufacturing of seen mild which is measured in a luminometer. The quantity of mild generated is immediately proportional to the quantity of gonococcal goal rRNA current in the pattern.

A complete of 407 medical specimens (203 urethral and 204 endocervical) had been collected from high-risk walk-in sufferers attending a sexually transmitted illness clinic.

Separate affected person specimens had been collected for tradition on Martin-Lewis medium in JEMBEC plates and for DNA probe assay. Statistical evaluation of the total comparative outcomes confirmed that the DNA probe assay had a sensitivity, specificity, and constructive and destructive predictive values of 93, 99, 97, and 99%, respectively, in a affected person inhabitants with a gonococcal illness prevalence of 21%.

Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.
Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

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The outcomes of this comparative examine confirmed that the prototype chemiluminescent DNA probe assay is a fast and dependable noncultural various for the laboratory diagnosis of gonorrhea.

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